Multiplex reverse transcriptase PCR assay for simultaneous detection of three fish viruses.
نویسندگان
چکیده
A multiplex reverse transcriptase (RT)-PCR assay was developed for the simultaneous detection of three different fish viruses: infectious pancreatic necrosis virus (IPNV), infectious hematopoietic necrosis virus (IHNV), and viral hemorrhagic septicemia virus (VHSV). The sensitivity levels of the multiplex RT-PCR assay were 100, 1, and 32 50% tissue culture infective doses/ml for IPNV, IHNV, and VHSV, respectively.
منابع مشابه
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عنوان ژورنال:
- Journal of clinical microbiology
دوره 37 12 شماره
صفحات -
تاریخ انتشار 1999